English: Estradiol levels across the menstrual cycle in 36 normally cycling and ovulatory premenopausal women based on 956 specimens. The horizontal lines are the mean integrated values for each curve. The vertical line is mid-cycle. Determinations were done using chemiluminescent microparticle immunoassay (CMIA).

Description from the source:

"The expected ranges for the ARCHITECT Estradiol assay were obtained by testing specimens drawn from 101 males, 72 postmenopausal females and normal menstruating females. For the normal menstruating female ranges, specimens were obtained from 36 women drawn throughout their cycle, resulting in a total of 956 specimens. Variations in cycle length were normalized by aligning the cycles based on Day 0 as the day of the LH peak (same day as the FSH peak and same day or one day after the estradiol peak). To establish cycle-specific reference ranges, the specimens were categorized as follicular phase, mid-cycle phase and luteal phase. Follicular phase was defined as the period of time from 15 days to 2 days prior (-15 to -2) to the period of the mid-cycle gonadotropin surge (Days -1 to +1). The luteal phase was defined as +2 days to +15 days.17 All cycles included in establishing reference ranges were ovulatory."

Sources of the values:

• Abbott (2009). ARCHITECT Estradiol Assay (PDF). Archived from the original on 2020-01-27. Retrieved on 2020-04-30.
• (2006). "Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT analyzer". Clin. Chem. Lab. Med. 44 (7): 883–7. DOI:10.1515/CCLM.2006.160. PMID 16776638.
• (February 2008). "Mass spectrometric and physiological validation of a sensitive, automated, direct immunoassay for serum estradiol using the Architect". Clin. Chim. Acta 388 (1-2): 99–105. DOI:10.1016/j.cca.2007.10.020. PMID 18023274.

The first source also contains a table with n, median values, and 95% ranges for the different phases of the menstrual cycle (follicular, mid-cycle, and luteal).