View source for DnaA

{{Short description|Protein}}
{{Infobox nonhuman protein
| Name = Chromosomal replication initiator protein dnaA
| image = 
| width = 
| caption = 
| Organism = ''[[Escherichia coli]]''<br/>(str. K-12 substr. MG1655)
| TaxID = 511145
| Symbol = DnaA
| AltSymbols = 
| EntrezGene = 948217
| PDB = 
| RefSeqmRNA = 
| RefSeqProtein = NP_418157.1
| UniProt = P03004
| ECnumber = 
| Chromosome = genome
| EntrezChromosome = NC_000913.2
| GenLoc_start = 3880138
| GenLoc_end = 3881961
}}
{{Infobox protein family
| Symbol = Bac_DnaA_C
| Name = Bac_DnaA_C
| image = PDB 1j1v EBI.jpg
| width = 
| caption = crystal structure of dnaa domainiv complexed with dnaabox dna
| Pfam = PF08299
| Pfam_clan = CL0123 
| InterPro = IPR013159
| SMART = 
| PROSITE = 
| MEROPS = 
| SCOP = 1j1v
| TCDB = 
| OPM family = 
| OPM protein = 
| CAZy = 
| CDD = 
}}
'''DnaA''' is a protein that activates initiation of DNA replication in bacteria.<ref name="foster">{{cite book |title=Microbiology: an evolving science |vauthors=Foster JB, Slonczewski J |publisher=W.W. Norton & Co. |year=2009 |isbn=978-0-393-97857-5 |location=New York}}</ref> Based on the Replicon Model, a positively active initiator molecule contacts with a particular spot on a circular chromosome called the replicator to start DNA replication.<ref name="Hansen 319">{{Cite journal |last1=Hansen |first1=Flemming G. |last2=Atlung |first2=Tove |date=2018-02-28 |title=The DnaA Tale |journal=Frontiers in Microbiology |volume=9 |page=319 |doi=10.3389/fmicb.2018.00319 |issn=1664-302X |pmc=5835720 |pmid=29541066 |doi-access=free}}</ref> It is a replication initiation factor which promotes the unwinding of DNA at oriC.<ref name="foster" /> The DnaA proteins found in all bacteria engage with the DnaA boxes to start chromosomal replication. The onset of the initiation phase of DNA replication is determined by the concentration of DnaA.<ref name="foster" /> DnaA accumulates during growth and then triggers the initiation of replication.<ref name="foster" /> Replication begins with active DnaA binding to 9-mer (9-bp) repeats upstream of oriC.<ref name="foster" /> Binding of DnaA leads to strand separation at the 13-mer repeats.<ref name="foster" /> This binding causes the DNA to loop in preparation for melting open by the helicase DnaB.<ref name="foster" />{{Infobox protein family
| Symbol = Bac_DnaA
| Name = Bac_DnaA
| image = PDB 2hcb EBI.jpg
| width = 
| caption = structure of amppcp-bound dnaa from aquifex aeolicus
| Pfam = PF00308
| Pfam_clan = CL0023 
| InterPro = IPR013317
| SMART = 
| PROSITE = PDOC00771
| MEROPS = 
| SCOP = 1j1v
| TCDB = 
| OPM family = 
| OPM protein = 
| CAZy = 
| CDD = 
}}

== Function ==
DnaA consists mainly in two different forms, the active [[Adenosine triphosphate|ATP]]-form and the inactive [[Adenosine diphosphate|ADP]].<ref name="foster"/><ref name="Hansen-2018" /> The level of active DnaA within a cell is low immediately after a cell has divided.<ref name="foster"/> Although the active form of DnaA requires ATP, the formation of the ''oriC''/DnaA complex and subsequent DNA unwinding does not require [[ATP hydrolysis]].<ref name="pmid21035377">{{cite journal | vauthors = Leonard AC, Grimwade JE | title = Regulating DnaA complex assembly: it is time to fill the gaps | journal = Current Opinion in Microbiology | volume = 13 | issue = 6 | pages = 766–772 | date = December 2010 | pmid = 21035377 | pmc = 3005629 | doi = 10.1016/j.mib.2010.10.001 }}</ref>

The ''oriC'' site in ''[[E. coli]]'' has three [[adenosine|A]][[thymine|T]] rich 13 [[base pair]] regions ([[DNA unwinding element|DUEs]])  followed by four 9 bp regions with the sequence TTAT([[cytosine|C]] or A)CA(C or A)A.<ref name="pmid6091903">{{cite journal | vauthors = Fuller RS, Funnell BE, Kornberg A | title = The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites | journal = Cell | volume = 38 | issue = 3 | pages = 889–900 | date = October 1984 | pmid = 6091903 | doi = 10.1016/0092-8674(84)90284-8 | s2cid = 23316215 }}</ref> DnaA [[molecule]]s bind to the 9 bp regions, which wrap around the proteins causing the [[DNA]] at the AT-rich region to unwind.<ref name=":0">{{Cite journal |last1=Grimwade |first1=Julia E. |last2=Leonard |first2=Alan C. |date=2021-09-20 |title=Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During the Escherichia coli Cell Cycle by Transcriptional Modulators That Interact With Origin DNA |journal=Frontiers in Microbiology |volume=12 |doi=10.3389/fmicb.2021.732270 |doi-access=free |issn=1664-302X |pmc=8488378 |pmid=34616385}}</ref> There are currently 11 DnaA binding sites identified within ''oriC'', to which DnaA binds with differential affinity.<ref name=":0" /> When DNA replication is about to commence, DnaA occupies all of the high and low affinity binding sites. The denatured AT-rich region allows for the recruitment of [[DnaB helicase|DnaB]] ([[helicase]]), which complexes with [[DnaC]] (helicase loader). DnaC helps the helicase to bind to and to properly accommodate the [[ssDNA]] at the 13 bp region; this is accomplished by [[Adenosine triphosphate|ATP]] [[hydrolysis]], after which DnaC is released. [[Single-strand binding protein]]s (SSBs) stabilize the single DNA strands in order to maintain the [[replication bubble]]. [[DnaB helicase|DnaB]] is a 5'→3' helicase, so it travels on the [[lagging strand]]. It associates with [[DnaG]] (a [[primase]]) to form the only [[Primer (molecular biology)|primer]] for the [[leading strand]] and to add [[RNA]] primers on the lagging strand. The interaction between DnaG and DnaB is necessary to control the longitude of Okazaki fragments on the lagging strand. [[DNA polymerase III]] is then able to start [[DNA replication]].

DnaA is made up of four domains: the first is the N-terminal that associates with regulatory proteins, the second is a helical linker region, the third domain is a AAA+ region that binds to ATP, and the fourth domain is the C-terminal DNA binding region.<ref>{{cite journal | vauthors = Costa A, Hood IV, Berger JM | title = Mechanisms for initiating cellular DNA replication | journal = Annual Review of Biochemistry | volume = 82 | pages = 25–54 | date = 2013-01-01 | pmid = 23746253 | pmc = 4696014 | doi = 10.1146/annurev-biochem-052610-094414 }}</ref> DnaA contains two [[conserved sequence|conserved]] regions: the first is located in the central part of the protein and corresponds to the ATP-binding domain, the second is located in the C-terminal half and is involved in DNA-binding.<ref name="pmid7744016">{{cite journal | vauthors = Roth A, Messer W | title = The DNA binding domain of the initiator protein DnaA | journal = The EMBO Journal | volume = 14 | issue = 9 | pages = 2106–2111 | date = May 1995 | pmid = 7744016 | pmc = 398312 | doi = 10.1002/j.1460-2075.1995.tb07202.x }}</ref>

== DnaA mutants ==
The first strains to have the dnaA gene mutated were the temperature-sensitive K-12 strains CRT46 and CRT83, with the corresponding strain numbers beingdnaA46 and dnaA83. In contrary to dnaA mutants, the PC2 strain has a mutation in the dnaC gene, which codes for the loading factor for the DNA helicase dnaB.<ref>{{Cite journal |last1=Ricard |first1=Matthieu |last2=Hirota |first2=Yukinori |date=October 1973 |title=Process of Cellular Division in Escherichia coli: Physiological Study on Thermosensitive Mutants Defective in Cell Division |journal=Journal of Bacteriology |language=en |volume=116 |issue=1 |pages=314–322 |doi=10.1128/jb.116.1.314-322.1973 |pmid=4583216 |pmc=246424 |issn=0021-9193}}</ref>

== Synthesis ==
DnaA has the ability to bind its own [[Promoter (genetics)|promoter]]. When DnaA binds to its own promoter it blocks [[RNA polymerase]] from binding the [[Promoter (genetics)|promoter]] and inhibits initiation of [[Transcription (biology)|transcription]]. In this way, DnaA is able to regulate its own expression.<ref name="Hansen-2018">{{cite journal | vauthors = Hansen FG, Atlung T | title = The DnaA Tale | journal = Frontiers in Microbiology | volume = 9 | page = 319 | date = 2018-02-28 | pmid = 29541066 | pmc = 5835720 | doi = 10.3389/fmicb.2018.00319 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Speck C, Weigel C, Messer W | title = ATP- and ADP-dnaA protein, a molecular switch in gene regulation | journal = The EMBO Journal | volume = 18 | issue = 21 | pages = 6169–6176 | date = November 1999 | pmid = 10545126 | pmc = 1171680 | doi = 10.1093/emboj/18.21.6169 }}</ref> This process is called [[autoregulation]].<ref name="Braun 159–169">{{Cite journal |last1=Braun |first1=Robert E. |last2=O'Day |first2=Kathy |last3=Wright |first3=Andrew |date=January 1985 |title=Autoregulation of the DNA replication gene dnaA in E. coli K-12 |url=https://linkinghub.elsevier.com/retrieve/pii/0092867485903198 |journal=Cell |language=en |volume=40 |issue=1 |pages=159–169 |doi=10.1016/0092-8674(85)90319-8|pmid=2981626 |s2cid=10594994 |url-access=subscription }}</ref>

== Regulation ==
Each cell division cycle triggers a new round of chromosome replication with the accumulation of DnaA, the initiator protein, on the OriC region of DNA.<ref>{{Cite journal |last1=Grimwade |first1=Julia E |last2=Rozgaja |first2=Tania A |last3=Gupta |first3=Rajat |last4=Dyson |first4=Kyle |last5=Rao |first5=Prassanna |last6=Leonard |first6=Alan C |date=2018-07-06 |title=Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication |url=https://academic.oup.com/nar/article/46/12/6140/5003459 |journal=Nucleic Acids Research |language=en |volume=46 |issue=12 |pages=6140–6151 |doi=10.1093/nar/gky457 |issn=0305-1048 |pmc=6158602 |pmid=29800247}}</ref> It is crucial to regulate DnaA-ATP monomer interactions with oriC during helicase loading and unwinding of origin DNA for precise timing. DnaA recognition sites in Escherichia coli are arranged in OriC to facilitate staged pre-replication complex assembling, with DnaA interacting with low affinity sites as it oligomerizes to fill the gaps between high affinity sites as it oligomerizes. There may be numerous gap-filling strategies to link OriC functions to bacterial lifestyles in nature, which may account for the wide variability of OriC DnaA recognition site patterns.<ref name="pmid21035377" /> The two forms of DnaA, the active [[Adenosine triphosphate|ATP]]- and [[Adenosine diphosphate|ADP]]-form are regulated. The [[Adenosine triphosphate|ATP]]-form is converted to the [[Adenosine diphosphate|ADP]]-form through either Regulatory inactivation of DnaA ([[RIDA]]),<ref>{{cite journal |vauthors=Katayama T, Kubota T, Kurokawa K, Crooke E, Sekimizu K |date=July 1998 |title=The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E. coli chromosomal replicase |journal=Cell |volume=94 |issue=1 |pages=61–71 |doi=10.1016/S0092-8674(00)81222-2 |pmid=9674428 |s2cid=15215988 |doi-access=free}}</ref> which in turn consists of the [[Hda]] protein and the β sliding clamp ([[DnaN]])<ref>{{cite journal |vauthors=Kato J, Katayama T |date=August 2001 |title=Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli |journal=The EMBO Journal |volume=20 |issue=15 |pages=4253–4262 |doi=10.1093/emboj/20.15.4253 |pmc=149159 |pmid=11483528}}</ref> and ''[[datA]]-''dependent DnaA-ATP hydrolysis.<ref>{{cite journal |vauthors=Kasho K, Katayama T |date=January 2013 |title=DnaA binding locus datA promotes DnaA-ATP hydrolysis to enable cell cycle-coordinated replication initiation |journal=Proceedings of the National Academy of Sciences of the United States of America |volume=110 |issue=3 |pages=936–941 |bibcode=2013PNAS..110..936K |doi=10.1073/pnas.1212070110 |pmc=3549119 |pmid=23277577 |doi-access=free}}</ref> The ADP-form is converted to the ATP-form by [[DnaA-reactivating sequences]] 1 and 2 (DARS1 and DARS2).<ref>{{cite journal |vauthors=Fujimitsu K, Senriuchi T, Katayama T |date=May 2009 |title=Specific genomic sequences of E. coli promote replicational initiation by directly reactivating ADP-DnaA |journal=Genes & Development |volume=23 |issue=10 |pages=1221–1233 |doi=10.1101/gad.1775809 |pmc=2685538 |pmid=19401329}}</ref> 

'''''Regulation of DnaA binding to DNA at OriC''''' 

Since DNA replication must occur irreversibly and only once per cycle, the binding behavior of DnaA complexes to OriC is a highly regulated, and therefore dependent on many other cellular mechanisms.<ref name=":3" /><ref name=":7" /> While all OriC sites are bound at replication initiation, there are three high-affinity binding sites–R1, R2, and R4–that are typically occupied by DnaA for the majority of the cell cycle, thus making their binding somewhat less dependent of other events happening within the cell at a given point in time.<ref name="pmid21035377" /><ref name=":1">{{Cite journal |last1=Nievera |first1=Christian |last2=Torgue |first2=Julien J.C. |last3=Grimwade |first3=Julia E. |last4=Leonard |first4=Alan C. |date=November 2006 |title=SeqA Blocking of DnaA-oriC Interactions Ensures Staged Assembly of the E. coli Pre-RC |journal=Molecular Cell |language=en |volume=24 |issue=4 |pages=581–592 |doi=10.1016/j.molcel.2006.09.016 |pmc=1939805 |pmid=17114060}}</ref> By contrast, the lower affinity sites are typically only bound to DnaA complexes right before replication begins.<ref name=":0" /> There are currently eight identified sites with lower DnaA/OriC binding affinity: R5 (or R5M), I1, I2, I3 and R3, tau2, C1, C2 and C3.<ref>{{Cite journal |last1=Leonard |first1=Alan C. |last2=Grimwade |first2=Julia E. |date=2015-06-02 |title=The orisome: structure and function |journal=Frontiers in Microbiology |language=English |volume=6 |page=545 |doi=10.3389/fmicb.2015.00545 |doi-access=free |issn=1664-302X |pmc=4451416 |pmid=26082765}}</ref> Between the R1 and R2 high affinity sites exist the R5M, tau2, I1, and I2 low affinity sites, and C3, C2, 13, and C1 exist between the R2 and R4 sites.<ref name=":0" /> The I sites, tau2, C2, and C3 sites preferentially bind with and are more efficient at binding to DnaA in its ATP-bound active form (DnaA-ATP) prior to DNA strand separation, whereas the R1-R5 sites and C1 site have not demonstrated a preference for binding with DnaA-ATP over DnaA-ADP.<ref name=":2">{{Cite journal |last1=Grimwade |first1=Julia E |last2=Rozgaja |first2=Tania A |last3=Gupta |first3=Rajat |last4=Dyson |first4=Kyle |last5=Rao |first5=Prassanna |last6=Leonard |first6=Alan C |date=2018-07-06 |title=Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication |url=https://academic.oup.com/nar/article/46/12/6140/5003459 |journal=Nucleic Acids Research |language=en |volume=46 |issue=12 |pages=6140–6151 |doi=10.1093/nar/gky457 |issn=0305-1048 |pmc=6158602 |pmid=29800247}}</ref> OriC binding with active DnaA-ATP complexes at the lower affinity I sites, as well as the tau2, C2, and C3, sites is required for the strand separation process to initiate in a time regulated manner, meaning DnaA-ATP cannot be substituted with inactive DnaA-ADP complexes to initiate replication properly and with sufficient regulation.<ref name=":0" /> Recent studies suggest that while OriC sites bound entirely to DnaA-ADP complexes are capable of preparing the cell for DNA replication, they struggle to maintain the healthy and consistent replication frequency regulation cells continuing OriC sites bound to DnaA-ATP complexes achieve, perhaps explaining why some sites bind preferentially to the active DnaA conformation over the inactive conformation.<ref name=":0" /><ref name=":2" /> Two other proteins, an integration host factor (IHF) protein and a DnaA initiator associating (DiaA) protein, help facilitate the binding of DnaA-ATP complexes to the OriC sites and set the stage for replication initiation to occur.<ref name=":3">{{Cite journal |last1=Katayama |first1=Tsutomu |last2=Ozaki |first2=Shogo |last3=Keyamura |first3=Kenji |last4=Fujimitsu |first4=Kazuyuki |date=March 2010 |title=Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC |url=https://www.nature.com/articles/nrmicro2314 |journal=Nature Reviews Microbiology |language=en |volume=8 |issue=3 |pages=163–170 |doi=10.1038/nrmicro2314 |pmid=20157337 |issn=1740-1534|url-access=subscription }}</ref><ref name=":4">{{Cite journal |last1=Sugiyama |first1=Ryo |last2=Kasho |first2=Kazutoshi |last3=Miyoshi |first3=Kenya |last4=Ozaki |first4=Shogo |last5=Kagawa |first5=Wataru |last6=Kurumizaka |first6=Hitoshi |last7=Katayama |first7=Tsutomu |date=2019-12-02 |title=A novel mode of DnaA–DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity |url=https://academic.oup.com/nar/article/47/21/11209/5571582 |journal=Nucleic Acids Research |language=en |volume=47 |issue=21 |pages=11209–11224 |doi=10.1093/nar/gkz795 |issn=0305-1048 |pmc=6868365 |pmid=31535134}}</ref><ref name=":5">{{Cite journal |last1=Kasho |first1=Kazutoshi |last2=Ozaki |first2=Shogo |last3=Katayama |first3=Tsutomu |date=2023-07-18 |title=IHF and Fis as Escherichia coli Cell Cycle Regulators: Activation of the Replication Origin oriC and the Regulatory Cycle of the DnaA Initiator |journal=International Journal of Molecular Sciences |language=en |volume=24 |issue=14 |article-number=11572 |doi=10.3390/ijms241411572 |doi-access=free |pmid=37511331 |pmc=10380432 |issn=1422-0067}}</ref>

IHF plays a key functional role positively regulating the binding of DnaA complexes to the lower affinity OriC sites as the cell prepares for replication, essentially evening the playing field between the high and low affinity OriC sites in terms of their ability to bind with DnaA complexes.<ref name=":6">{{Cite journal |last1=Grimwade |first1=Julia E. |last2=Ryan |first2=Valorie T. |last3=Leonard |first3=Alan C. |date=2002 |title=IHF redistributes bound initiator protein, DnaA, on supercoiled oriC of Escherichia coli |url=https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2000.01755.x |journal=Molecular Microbiology |language=en |volume=35 |issue=4 |pages=835–844 |doi=10.1046/j.1365-2958.2000.01755.x |pmid=10692160 |issn=1365-2958|url-access=subscription }}</ref> Cooperative binding is thought to be a mechanism in which the high-affinity sites supply the lower-affinity sites within their vicinity with DnaA-ATP complexes in the moments leading up to replication initiation.<ref name=":0" /> While DnaA can saturate all OriC binding sites in systems lacking IHF, a much higher concentration of DnaA is needed in the cellular environment for this to be achieved.<ref name=":6" /> However, in these situations, cells also experience a loss of synchronization in their replication initiation timing, indicating how important IHF is for maintaining consistent regulation of this process in cells and preventing a lag in the initiation of replication.<ref name=":0" /><ref name=":6" /> When IHF is present in a cellular system, IHF enhances DnaA binding to low affinity OriC sites without any need for increasing the baseline concentration of DnaA present, further highlighting its importance in maintaining replication initiation timing.<ref name=":0" /><ref name=":6" /> 

Conformationally, IHF assists in promoting the process of DnaA-ATP complexes binding to the low affinity OriC binding sites at the right time by binding to a different site on OriC ahead of replication initiation, causing DNA it to bend in such a way that facilitates efficient binding with DnaA-ATP complexes.<ref name=":1" /><ref name=":7">{{Cite journal |last1=Leonard |first1=Alan C. |last2=Grimwade |first2=Julia E. |date=2005 |title=Building a bacterial orisome: emergence of new regulatory features for replication origin unwinding |journal=Molecular Microbiology |language=en |volume=55 |issue=4 |pages=978–985 |doi=10.1111/j.1365-2958.2004.04467.x |issn=1365-2958 |pmc=1400601 |pmid=15686547}}</ref>  Prior to IHF binding to OriC, a different protein, factor for inversion stimulation (FIS) protein, is bound to DNA for the majority of the cell cycle (with the exception of the events leading up to replication initiation), inhibiting the binding of IHF to DNA.<ref name=":1" /><ref name=":7" /><ref name=":8">{{Cite journal |last1=Cassler |first1=M. R. |last2=Grimwade |first2=J. E. |last3=Leonard |first3=A. C. |date=1995-12-01 |title=Cell cycle-specific changes in nucleoprotein complexes at a chromosomal replication origin. |journal=The EMBO Journal |volume=14 |issue=23 |pages=5833–5841 |doi=10.1002/j.1460-2075.1995.tb00271.x |issn=0261-4189 |pmc=394701 |pmid=8846776}}</ref> Consequently, the binding of DnaA complexes to the lower affinity OriC sites is also inhibited, thus, preventing the chromosomal replication process from starting prematurely and thereby demonstrating how FIS positively regulates the maintenance of a consistent cell cycle progression via inhibition.<ref name=":0" /><ref name=":1" /><ref name=":7" /><ref name=":8" /> As FIS binding to OriC weakens, IHF begins to bind to OriC, therefore increasing the low affinity sites' ability to bind to DnaA-ATP complexes, concurrent with IHF binding.<ref name=":7" /> The switch from FIS binding to IHF binding to DNA is hypothesized to be brought about by the generation of more DnaA-ATP complexes, promoted by the existence of the high affinity sites bound to DnaA while FIS is concurrently bound to DNA, which are then recruited to the high affinity region and build up, exerting a conformational stress on bound FIS (especially by accumulation at the R2 site, as it is closest to the FIS binding site), thereby deteriorating its binding ability with DNA.<ref name=":0" /><ref name=":7" /> As a result, IHF can take advantage of the weakened state of FIS binding so that it can then bind to its own respective OriC site, causing DNA to bend and essentially align the accumulated DnaA-ATP complexes better with the low affinity binding sites, thus aiding in the facilitation of their binding with DnaA-ATP.<ref name=":7" /> In the absence of the switch-like behavior that occurs with the transition from FIS to IHF binding to DNA, cells are unable to maintain a control over the sequence of events that ensure replication initiation happens both irreversibly and only once per cell cycle.<ref name=":0" /> 

DiaA positively regulates the replication initiation timeline by facilitating the binding of DnaA-ATP complexes on OriC sites.<ref name=":4" /><ref name=":9">{{Cite journal |last1=Keyamura |first1=Kenji |last2=Abe |first2=Yoshito |last3=Higashi |first3=Masahiro |last4=Ueda |first4=Tadashi |last5=Katayama |first5=Tsutomu |date=2009-09-11 |title=DiaA Dynamics Are Coupled with Changes in Initial Origin Complexes Leading to Helicase Loading |journal=Journal of Biological Chemistry |language=en |volume=284 |issue=37 |pages=25038–25050 |doi=10.1074/jbc.M109.002717 |doi-access=free |pmc=2757208 |pmid=19632993}}</ref> DiaA binds to DnaA in its tetrameric form (consisting of four DiaA protomers (individual proteins) bound to one another), specifically to the first domain of DnaA–in the same region where another protein, replicative DNA helicase (DnaB), is presumed to bind with DnaA.<ref name=":3" /><ref name=":9" /> Due to its tetrameric structure, DiaA has the ability to bind to multiple DnaA-ATP complexes at a time, as each protamer within the homotetramer consists of an DnaA-ATP binding site.<ref name=":9" /> This beneficial characteristic of DiaA tetramers can aid in promoting the cooperative binding behavior of transferring DnaA-ATP molecules to different sites on the OriC region of DNA as the cell prepares to undergo chromosomal replication.<ref name=":9" /> DiaA also negatively regulates the chromosomal replication process by inhibiting the binding of the DnaB protein, whose presence and function is required for chromosomal replication, from binding to DnaA-ATP complexes assembled on OriC, therefore helping to preserve the inflexible regulation sequence of events needed for a controlled replication process and prevents asynchronous initiation within the overall cycle cycle.<ref name=":3" /><ref name=":9" /> Thus, taken together, IHF and DiaA, along with the proteins they interact with in their respective binding mechanisms, are very both important for helping DnaA-ATP complexes bind to all the identified binding sites on OriC, including the low affinity sites, within a timely manner that ensures replication initiation occurs irreversibly and only a single time during the cell cycle.<ref name=":5" /> 

Once replication initiation has occurred and DNA has undergone strand separation successfully, a different process commences to make sure DnaA-ATP cannot bind directly to DNA again with a protein that negatively regulates replication initiation–the locus of datA–SeqA.<ref name=":3" /><ref name=":1" />  When DNA unwinds post-initiation, new replication forks are generated, a process that subsequently leads to the unbinding of DnaA complexes from the OriC sites.<ref name=":0" /><ref name=":10">{{Cite journal |last=Jha |first=Jyoti K. |last2=Chattoraj |first2=Dhruba K. |date=2016-12-08 |editor-last=Marinus |editor-first=Martin G. |title=Inactivation of Individual SeqA Binding Sites of the E. coli Origin Reveals Robustness of Replication Initiation Synchrony |url=https://dx.plos.org/10.1371/journal.pone.0166722 |journal=PLoS One |language=en |volume=11 |issue=12 |article-number=e0166722 |doi=10.1371/journal.pone.0166722 |issn=1932-6203 |pmc=5145175 |pmid=27930658 |doi-access=free}}</ref> DNA's GATC sites within OriC and at the region where the ''dnaA'' promoter exists become hemimethylated, and therefore experience a reduced ability to function and express the same way as they would while methylated.<ref name=":0" /><ref name=":10" /> SeqA is able to physically prevent replication from starting up again prematurely by binding to the hemimethylated GATA sites on OriC–many of which somewhat overlap with a couple of the low affinity binding DnaA binding sites, as well as IHF's binding site on OriC–essentially shielding IHF and DnaA from binding to OriC.<ref name=":0" /><ref name=":10" /> However, the high affinity OriC DnaA complex binding sites are not blocked by SeqA binding to DNA, thus explaining how DnaA stays bound to the three high affinity sites throughout the majority of the cycle cycle duration.<ref name=":0" /> When GATC sites are bound to SeqA while hemimethylated, they are limited in their ability to synthesize new DnaA proteins as well, thus causing DnaA concentration within the cell to decline post initiation.<ref name=":0" /> Thus, with these sites blocked by SeqA, DnaA-ATP binding to some of the lower affinity sites is not possible for a combination of reasons.<ref name=":0" /><ref name=":3" /> In studies performed with strains lacking the ability to produce SeqA, cells were unable to synchronously initiate replication once per cycle, mirroring the effects of what happens when cells lack IHF.<ref name=":1" /><ref name=":10" /> Since the binding of the low affinity sites on OriC are basically the key event that kick start replication initiation and DNA's unraveling, SeqA's blocking of DnaA-ATP complex binding during the majority of the cell cycle is vital for keep cells healthy by maintaining a consistent cycle.<ref name=":10" />

== DnaA protein structure ==
There are four disciplines within the DnaA protein. An initial comparison of ''Escherichia coli'' and ''Bacillus subtilis'' proteins led to the discovery of a sphere structure, which revealed a relatively conserved N-terminus and a largely conserved large C-terminus separated by a region that was mostly variable.<ref>{{Cite journal |last1=Michelsen |first1=Ole |last2=Teixeira de Mattos |first2=M. Joost |last3=Jensen |first3=Peter Ruhdal |last4=Hansen |first4=Flemming G. |date=2003-04-01 |title=Precise determinations of C and D periods by flow cytometry in Escherichia coli K-12 and B/r |journal=Microbiology |language=en |volume=149 |issue=4 |pages=1001–1010 |doi=10.1099/mic.0.26058-0 |pmid=12686642 |issn=1350-0872|doi-access=free }}</ref> As an example, the Enterobacterial proteins have nearly identical N- and C-terminal sequences, however they are characterized by numerous amino acid adjustments, elisions, and insertions in the variable regions.<ref name="Morigen 349–362">{{Cite journal |last1=Morigen |last2=Løbner-Olesen |first2=Anders |last3=Skarstad |first3=Kirsten |date=2003-08-22 |title=Titration of the Escherichia coli DnaA protein to excess datA sites causes destabilization of replication forks, delayed replication initiation and delayed cell division: Destabilization of replication forks by excess datA |journal=Molecular Microbiology |language=en |volume=50 |issue=1 |pages=349–362 |doi=10.1046/j.1365-2958.2003.03695.x|pmid=14507385 |s2cid=21606647 |doi-access=free }}</ref> There is an AAA+ family ATPase motif and an independent DNA binding sphere in the C-terminal region. It was determined by NMR that Escherichia coli sphere IV had a crystal-clear structure when complexed with a DnaA- box. As a result, it was confirmed that the DNA list is intermediated by a combination of a helix-turn-helix motif and an introductory circle. When bound to ATP, but not to ADP, DnaA forms a super-helical structure with four monomers per turn. The structure of sphere I has been determined from three additional bacterial species and ''Escherichia coli'' by NMR.<ref>{{Cite journal |last1=Frimodt-Møller |first1=Jakob |last2=Charbon |first2=Godefroid |last3=Krogfelt |first3=Karen A. |last4=Løbner-Olesen |first4=Anders |date=2016-09-02 |editor-last=Viollier |editor-first=Patrick H. |title=DNA Replication Control Is Linked to Genomic Positioning of Control Regions in Escherichia coli |journal=PLOS Genetics |language=en |volume=12 |issue=9 |article-number=e1006286 |doi=10.1371/journal.pgen.1006286 |pmid=27589233 |pmc=5010248 |issn=1553-7404 |doi-access=free }}</ref>

== Autoregulation of DnaA protein synthesis ==
[[File:PDB_1pji_EBI.jpg|thumb|DnaA protein structure]]
The research on dnaA(Ts) mutants provided the first proof that the dnaA gene is autoregulated. DnaA protein is still produced at non-permissive temperatures where it is inactive, but in some mutants it can be made active again by returning to a temperature that is conducive to development.<ref name="Morigen 349–362"/> This reversible initiation capacity—which was larger than anticipated given the mass gain of the culture—could be seen in the absence of protein synthesis at the permissive temperature and suggested that the DnaA protein synthesis was derepressed at the high growth temperature. These results prompted a thorough investigation of the dnaA46 mutant under permissive, intermediate, and non-permissive development conditions.<ref name="Hansen 387–396">{{Cite journal |last1=Hansen |first1=Egon B. |last2=Atlung |first2=Tove |last3=Hansen |first3=Flemming G. |last4=Skovgaard |first4=Ole |last5=von Mevenburg |first5=Kaspar |date=September 1984 |title=Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli |url=http://link.springer.com/10.1007/BF00436184 |journal=Molecular and General Genetics |language=en |volume=196 |issue=3 |pages=387–396 |doi=10.1007/BF00436184 |pmid=6094968 |s2cid=3094956 |issn=0026-8925|url-access=subscription }}</ref> The study's findings revealed that as growth temperature increased, the DnaA46 protein's activity decreased, leading to progressively decreasing DNA and origin concentrations at intermediate temperatures. An increase in initiation capacity was seen concurrently with a decrease in DnaA protein activity. Hansen and Rasmussen (1977) argued that the DnaA protein had a positive effect in replication initiation aing transcripts entering the dnaA gene were found as a result of sequencing the dnaA promoter region and the dnaA gene.<ref name="Hansen 387–396"/> The DnaA promoter region has nine GATC sites within 225 base pairs, and a sequence that is similar to nd a negative role in its own synthesis based on these observations. Two promoters providrepetitions (DnaA-boxes) in the oriC region was found between the two promoters. According to several studies, the DnaA protein negatively regulates both promoters. In these research, it was discovered that the dnaA transcription was upregulated by 4- to 5-fold at non-permissive temperatures in dnaATs mutants and repressed by the same amount when DnaA protein was overproduced. The autoregulation of the dnaA gene requires the DnaA-box.<ref>{{Cite journal |last1=Atlung |first1=Tove |last2=Clausen |first2=Erik S. |last3=Hansen |first3=Flemming G. |date=August 1985 |title=Autoregulation of the dnaA gene of Escherichia coli K12 |url=http://link.springer.com/10.1007/BF00425729 |journal=Molecular and General Genetics |language=en |volume=200 |issue=3 |pages=442–450 |doi=10.1007/BF00425729 |pmid=2995766 |s2cid=7623820 |issn=0026-8925|url-access=subscription }}</ref> The sequence of the dnaA2p promoter region has some intriguing characteristics that can be seen more clearly. This promoter contains two GATC sites, one in the 10 sequence and the other in the 35 sequence, and both in vivo and in vitro, methylation increases transcription from this promoter by a factor of two. In addition, DnaA protein binds to regions upstream of the dnaA2p promoter with a high affinity.<ref name="Braun 159–169"/>

== See also ==
*[[Origin recognition complex]]

== References ==
{{Reflist}}

== Further reading ==
{{refbegin}}
* {{cite book | vauthors = Pratt CA, Voet D, Voet JG | title = Fundamentals of Biochemistry: Life at the Molecular Level | publisher = Wiley | location = New York | year = 2012 | isbn = 978-0-470-54784-7  }}
* {{cite book | vauthors = Cox M, Nelson DR | title = Lehninger Principles of Biochemistry | url = https://archive.org/details/lehningerprincip00lehn_1 | url-access = registration | publisher = W H Freeman & Co. (Sd) | year = 2008 | isbn = 978-1-4292-2416-1  }}
* {{cite journal | vauthors = Scholefield G, Errington J, Murray H | title = Soj/ParA stalls DNA replication by inhibiting helix formation of the initiator protein DnaA | journal = The EMBO Journal | volume = 31 | issue = 6 | pages = 1542–1555 | date = March 2012 | pmid = 22286949 | pmc = 3321191 | doi = 10.1038/emboj.2012.6 }}
{{refend}}

== External links ==
* {{MeshName|DnaA+protein,+Bacteria}}

{{DNA replication}}
{{InterPro content|IPR013159}}

{{InterPro content|IPR013317}}

[[Category:Biomolecules]]
[[Category:DNA replication]]