Tampa Bay Mutiny<ref group="R1 trade">#2: D.C. United → Tampa Bay Mutiny. 26 May 2000: Tampa Bay Mutiny acquired this first-round pick, another first-round selection (#6) in the 2001 SuperDraft, a second-round selection in the 2002 MLS SuperDraft, and midfielder John Maessner from D.C. United in exchange for forward Raúl Díaz Arce and forward Pete Marino.<ref group="ref" name="Maessner"/></ref>
D.C. United<ref group="R1 trade">#3: Columbus Crew → D.C. United. 5 February 2001: D.C. United acquired this first-round selection from Columbus Crew in exchange for the #10 pick in the 2001 SuperDraft and goalkeeper Tom Presthus.<ref group="ref" name="Pick60"/></ref>
D.C. United<ref group="R1 trade">#4: Miami Fusion → D.C. United. 3 February 2001: D.C. United acquired this first-round pick and midfielder Brian Kamler from Miami Fusion in exchange for defender Carlos Llamosa.<ref group="ref" name="Pick60"/></ref>
Dallas Burn<ref group="R1 trade">#5: Colorado Rapids → Dallas Burn. 6 February 2000: Dallas Burn acquired this first-round pick and a first-round selection (#5) in the 2000 MLS SuperDraft from Colorado Rapids in exchange for the #3 pick in the 2000 SuperDraft.<ref group="ref" name="Pick5"/></ref>
Tampa Bay Mutiny<ref group="R1 trade">#6: New England Revolution. → Colorado Rapids → D.C. United → Tampa Bay Mutiny. 26 May 2000: Tampa Bay Mutiny acquired this first-round pick, another first-round selection (#2) in the 2001 SuperDraft, a second-round selection in the 2002 MLS SuperDraft, and midfielder John Maessner from D.C. United in exchange for forward Raúl Díaz Arce and forward Pete Marino.<ref group="ref" name="Maessner"/> On 18 February 2000, D.C. had acquired this pick from Colorado Rapids in exchange for midfielder Jason Moore.<ref group="ref" name="JMoore"/> More information needed.</ref>
D.C. United<ref group="R1 trade">#8: Tampa Bay Mutiny → New England Revolution → San Jose Earthquakes → D.C. United. 4 February 2001: D.C. United acquired this first-round selection, the #10 pick in the 2001 SuperDraft, and forward Abdul Thompson Conteh from San Jose Earthquakes in exchange for defender Jeff Agoos.<ref group="ref" name="Pick60"/> On 30 May 2000, San Jose had acquired this pick, defender Mike Burns, and defender Dan Calichman from New England Revolution in exchange for defender Mauricio Wright.<ref group="ref" name="Wright"/> On 6 December 1999, New England had acquired this pick and midfielder Mauricio Ramos from Tampa Bay Mutiny in exchange for a player allocation.<ref group="ref" name="Pick30"/></ref>
Columbus Crew<ref group="R1 trade">#10: MetroStars → San Jose Earthquakes → D.C. United → Columbus Crew. 5 February 2001: Columbus Crew acquired this first-round selection and goalkeeper Tom Presthus from D.C. United in exchange for the #3 pick in the 2001 SuperDraft.<ref group="ref" name="Pick60"/> On 4 February 2001, D.C. United had acquired this selection, the #8 pick in the 2001 SuperDraft, and forward Abdul Thompson Conteh from San Jose Earthquakes in exchange for defender Jeff Agoos.<ref group="ref" name="Pick60"/> On 3 November 2000, San Jose had acquired this selection from MetroStars in exchange for a second-round selection in the 2001 SuperDraft and defender Francis Okaroh.<ref group="ref" name="Pick13"/></ref>
Los Angeles Galaxy <ref group="R1 trade">#11: Chicago Fire → Los Angeles Galaxy. 6 February 2000: Los Angeles Galaxy acquired this first-round pick and a first-round selection in the 2000 MLS SuperDraft from Chicago Fire in exchange for midfielder DaMarcus Beasley.<ref group="ref" name="Beasley"/></ref>
MetroStars<ref group="R2 trade">#13: San Jose Earthquakes → MetroStars. 3 November 2000: MetroStars acquired this second-round selection and defender Francis Okaroh from San Jose Earthquakes in exchange for a first-round selection in the 2001 SuperDraft.<ref group="ref" name="Pick13"/></ref>
Tampa Bay Mutiny<ref group="R2 trade">#14: D.C. United → San Jose Earthquakes → Tampa Bay Mutiny. 5 February 2001: Tampa Bay Mutiny acquired this second-round pick and a third-round selection (#34) in the 2001 SuperDraft from San Jose Earthquakes in exchange for midfielder Manny Lagos.<ref group="ref" name="Pick60"/> On 6 February 2000, San Jose had acquired this pick and a second-round selection in the 2000 MLS SuperDraft from D.C. United in exchange for a second-round pick in the 2000 SuperDraft.<ref group="ref" name="Quakes"/></ref>
D.C. United<ref group="R2 trade">#15: Columbus Crew → MetroStars → D.C. United. 4 February 2001: D.C. United acquired this second-round pick and goalkeeper Mike Ammann from MetroStars in exchange for a third-round selection (#26) in the 2001 SuperDraft, midfielder Richie Williams, and an allocation.<ref group="ref" name="Pick60"/> On 3 May 2000, MetroStars had acquired this pick from Columbus Crew in exchange for midfielder Miles Joseph.<ref group="ref" name="Joseph15"/></ref>
Los Angeles Galaxy<ref group="R2 trade">#16: Miami Fusion → Chicago Fire → San Jose Earthquakes → Los Angeles Galaxy. 4 February 2001: Los Angeles Galaxy acquired this second-round pick from San Jose Earthquakes in change for defender Zak Ibsen.<ref group="ref" name="Pick60"/> On 15 March 2000, San Jose had acquired this pick, another second-round selection (#20) in the 2001 SuperDraft, a first-round selection in the 2002 MLS SuperDraft, and future considerations from Chicago Fire in exchange for an allocation.<ref group="ref" name="Quakes"/> Chicago used the allocation to sign Hristo Stoichkov.<ref group="ref" name="Stoichkov"/> On 10 March 2000, Chicago had acquired this pick and a conditional draft pick from Miami Fusion in exchange for defender Francis Okaroh.<ref group="ref" name="Pick35"/> It is believed that the conditions to activate the conditional draft pick were not met.</ref>
Miami Fusion<ref group="R2 trade">#18: Pick originally belonged to New England Revolution. → Colorado Rapids → D.C. United → Miami Fusion. 5 February 2001: Miami Fusion acquired this second-round pick from D.C. United in exchange for a second-round selection in the 2002 MLS SuperDraft. Earlier the same day, D.C. had acquired this pick from Colorado Rapids in exchange for a third-round pick (#36) in the 2001 SuperDraft and defender Geoff Aunger.<ref group="ref" name="Pick60"/> More information needed.</ref>
San Jose Earthquakes<ref group="R2 trade">#20: Pick originally belonged to Tampa Bay Mutiny. → Chicago Fire → San Jose Earthquakes. 15 March 2000: San Jose Earthquakes acquired this second-round pick, another second-round selection (#16) in the 2001 SuperDraft, a first-round selection in the 2002 MLS SuperDraft, and future considerations from Chicago Fire in exchange for an allocation.<ref group="ref" name="Quakes"/> Chicago used the allocation to sign Hristo Stoichkov.<ref group="ref" name="Stoichkov"/> More information needed.</ref>
New England Revolution<ref group="R2 trade">#21: Los Angeles Galaxy → Miami Fusion → New England Revolution. 8 July 2000: New England Revolution acquired this second-round selection, originally "future considerations", and forward Eric Wynalda from Miami Fusion in exchange for midfielder Ivan McKinley.<ref group="ref" name="Wynalda"/> The future considerations became this draft pick when Wynalda did not agree to a new contract with New England prior to the 2001 SuperDraft.<ref group="ref" name="Pick60"/> On 6 February 2000, Miami had acquired this pick and a third-round selection in the 2000 MLS SuperDraft from Los Angeles Galaxy in exchange for a third-round pick in the 2000 SuperDraft.<ref group="ref" name="Pick21"/></ref>
Los Angeles Galaxy<ref group="R2 trade">#22: MetroStars → Los Angeles Galaxy. 19 April 2000: Los Angeles Galaxy acquired this second-round selection from MetroStars in exchange for defender Steve Jolley.<ref group="ref" name="Jolley"/></ref>
Dallas Burn<ref group="R3 trade">#25: San Jose Earthquakes → Kansas City Wizards → Dallas Burn. 5 December 2000: Dallas Burn acquired this third-round selection from Kansas City Wizards in exchange for midfielder Mark Santel.<ref group="ref" name="Santel"/> On 3 May 2000, Kansas City had acquired this pick from San Jose Earthquakes in exchange for forward Harut Karapetyan.<ref group="ref" name="Quakes"/></ref>
MetroStars<ref group="R3 trade">#26: D.C. United → MetroStars. 4 February 2001: MetroStars acquired this third-round selection, midfielder Richie Williams, and an allocation from D.C. United in exchange for a second-round pick (#15) in the 2001 SuperDraft and goalkeeper Mike Ammann.<ref group="ref" name="Pick60"/></ref>
D.C. United<ref group="R3 trade">#28: Miami Fusion → D.C. United. 5 February 2001: D.C. United acquired this third-round pick from Miami Fusion in exchange for midfielder Antonio Otero and future considerations.<ref group="ref" name="Pick60"/></ref>
Chicago Fire<ref group="R3 trade">#29: Colorado Rapids → Chicago Fire. 10 April 2000: Chicago Fire acquired this third-round selection from Colorado Rapids in exchange for forward Junior Agogo.<ref group="ref" name="Pick35"/></ref>
Columbus Crew<ref group="R3 trade">#30: New England Revolution → Columbus Crew. 14 March 2000: Columbus Crew acquired this third-round selection and future considerations from New England Revolution in exchange for goalkeeper Juergen Sommer.<ref group="ref" name="Pick30"/></ref>
San Jose Earthquakes<ref group="R3 trade">#31: Dallas Burn → San Jose Earthquakes. 7 June 2000: San Jose Earthquakes acquired this third-round selection from Dallas Burn in exchange for forward Esmundo Rodriguez.<ref group="ref" name="Quakes"/></ref>
Colorado Rapids<ref group="R3 trade">#32: Tampa Bay Mutiny → Colorado Rapids. 19 April 2000: Colorado Rapids acquired this third-round pick and a second-round pick in the 2002 MLS SuperDraft from Tampa Bay Mutiny in exchange for midfielder Kevin Anderson.<ref group="ref" name="Anderson"/></ref>
Tampa Bay Mutiny<ref group="R3 trade">#34: Pick originally belonged to MetroStars. → San Jose Earthquakes → Tampa Bay Mutiny. 5 February 2001: Tampa Bay Mutiny acquired this third-round pick and a second-round selection (#14) in the 2001 SuperDraft from San Jose Earthquakes in exchange for midfielder Manny Lagos.<ref group="ref" name="Pick60"/> More information needed.</ref>
Kansas City Wizards<ref group="R3 trade">#35: Chicago Fire → Kansas City Wizards. 3 March 2000: Kansas City Wizards acquired this third-round pick and a fifth-round selection in the 2001 SuperDraft from Chicago Fire in exchange for goalkeeper Chris Snitko.<ref group="ref" name="Pick35"/></ref>
Colorado Rapids<ref group="R3 trade">#36: Kansas City Wizards → D.C. United → Colorado Rapids. 5 February 2001: Colorado Rapids acquired this third-round selection and defender Geoff Aunger from D.C. United in exchange for a second-round selection (#18) in the 2001 SuperDraft. Earlier the same day, D.C. had acquired this pick from Kansas City Wizards in exchange for forward A.J. Wood.<ref group="ref" name="Pick60"/></ref>
Miami Fusion<ref group="R4 trade">#38: D.C. United → Miami Fusion. 6 February 2000: Miami Fusion acquired this fourth-round selection and midfielder Keith Beach from D.C. United in exchange for a fourth-round pick in the 2000 MLS SuperDraft.<ref group="ref" name="Pick21"/></ref>
New England Revolution <ref group="R4 trade">#47: Chicago Fire → New England Revolution. 14 April 2000: New England Revolution acquired this fourth-round selection from Chicago Fire in exchange for midfielder Mike Sorber.<ref group="ref" name="Pick35"/></ref>
New England Revolution<ref group="R5 trade">#53: Colorado Rapids → New England Revolution. 16 March 2000: New England Revolution acquired this fifth-round selection from Colorado Rapids in exchange for midfielder Paul Dougherty.<ref group="ref" name="Dougherty"/></ref>
Kansas City Wizards <ref group="R5 trade">#59: Chicago Fire → Kansas City Wizards. 3 March 2000: Kansas City Wizards acquired this fifth-round pick and a third-round selection in the 2001 SuperDraft from Chicago Fire in exchange for goalkeeper Chris Snitko.<ref group="ref" name="Pick35"/></ref>
Tampa Bay Mutiny<ref group="R5 trade">#60: Kansas City Wizards → Tampa Bay Mutiny. 5 February 2001: Tampa Bay Mutiny acquired this fifth-round selection from Kansas City Wizards in exchange for a fifth-round pick in the 2002 MLS SuperDraft.<ref group="ref" name="Pick60"/></ref>
Chicago Fire<ref group="R6 trade">#68: Tampa Bay Mutiny → Chicago Fire. 6 February 2000: Chicago Fire acquired this sixth-round selection from Tampa Bay Mutiny in exchange for two sixth-round selections in the 2000 MLS SuperDraft.<ref group="ref" name="Pick21"/></ref>
Dallas Burn<ref group="R6 trade">#71: Chicago Fire → Dallas Burn.Date Needed: Dallas Burn acquired this sixth-round pick from Chicago Fire in exchange for a sixth-round selection in the 2002 MLS SuperDraft.<ref group="ref" name="Pick71"/></ref>
18 February 1999: Chicago Fire traded Zak Ibsen to Los Angeles Galaxy for 2001 second-round pick
24 February 1999, Colorado acquired GK Ian Feuer from New England Revolution in exchange for future considerations .<ref group="ref" name="Feuer"/> It is unclear whether this trade was fulfilled through a 2001 first-round pick (#6), a 2001 second-round pick (#18), or why New England gave up draft picks to unload a player.
2 August 1999, Tampa Bay Mutiny traded defender Sam George, midfielder Paul Dougherty, and a conditional draft choice to Chicago Fire in exchange for defender Richie Kotschau and midfielder Manny Lagos. The draft pick may be 2001 pick #20.<ref group="ref" name="Kotschau"/>