AFO

• Academia Film Olomouc, a Czech film festival
• Advanced Force Operations, a US military term
• Aero Force One, a fan club for the rock band Aerosmith
• Afghan Film Organization, a film production company and archive.
• Afo Dodoo (born 1973), Ghanaian footballer
• AFO Records, the first African-American owned record label in the US
• Afo, thought to be the world's oldest Clove Tree
• Alien Front Online, a video game
• Animal Face-Off, a television show
• Animal feeding operation, as defined by the Environmental Protection Agency
• Anime Festival Orlando, a yearly anime convention in Orlando, Florida
• Ankle-foot orthosis, a brace for the ankle and foot
• Anti-Fascist Organisation, a provisional organization in Burma during World War II
• Arellano-Felix Organization, a drug trafficking cartel in Mexico, also known as the Tijuana Cartel
• Assemblée de la francophonie de l'Ontario, a Canadian cultural organization
• Association of Field Ornithologists, an ornithological organization, publishers of Journal of Field Ornithology
• Australian Field Ornithology, a journal of BirdLife Australia
• Authorised Firearms Officer, a police officer in the UK who has received training and authorization to carry and use firearms
• Eloyi language (ISO-639: afo)
• Eloyi people, also called Afo
• A Funk Odyssey

Aviation

• Air Force One (disambiguation)
• IATA airport code for Afton Municipal Airport in State of Wyoming, United States