Amido black 10B

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Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.<ref>Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. Template:ISBN</ref> It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.<ref>Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. {{#invoke:CS1 identifiers|main|_template=doi}}</ref> Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

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