U-87 MG
In cell biology, U-87 MG is a human glioblastoma cell line that is commonly used in brain cancer research.<ref name=one>Template:Cite journal</ref>
History and Characteristics
Formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma), the U87 cell line has an epithelial morphology and was obtained from a 44-year-old female patient in 1966 at Uppsala University. The cell line was thought to be deposited at the Memorial Sloan Kettering Cancer Center in 1973, after which the ATCC obtained it in 1982.<ref>Template:Cite journal</ref> However, in 2016 the commonly used version of U87MG (from the ATCC) was found to be non-identical to its patient of origin.<ref name=":0">Template:Cite journal</ref> Analysis of DNA profile and mtDNA position suggest that the Uppsala U-87 MG line is authentic to the original tumor tissue while the ATCC U-87 MG line is not; The ATCC line is likely a bona fide human glioblastoma cell line of unknown origin.<ref name=":0" /> The entire sequence of the genome of U-87 MG has been published in PLoS Genetics.<ref name=one /> U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14 and it is reported that the cell line comes from a male patient of unknown age.<ref name="HTB-14">Template:Cite web</ref>
Growth conditions
U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/mL penicillin + 100 μg/mL streptomycin.<ref name="HTB-14"/> It is propagated at 37 °C in a 5% carbon dioxide atmosphere.