Sulforhodamine B

Template:Short description Template:Chembox Sulforhodamine B or Kiton Red 620 (C₂₇H₃₀N₂O₇S₂) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.<ref name="SCBT">Template:Cite web</ref>

Spectroscopy

The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.<ref name="SCBT" /> It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.<ref>Template:Cite journal</ref>

Applications

Sulforhodamine B is often used as a membrane-impermeable polar tracer<ref>Template:Cite journal</ref> or used for cell density determination via determination of cellular proteins (cytotoxicity assay).<ref>Template:Cite journal</ref>